Convergent synthesis and properties of photoactivable NADPH mimics targeting nitric oxide synthases

A new series of photoactivable NADPH mimics bearing one or two O-carboxymethyl groups on the adenosine moiety have been readily synthesized using click chemistry. These compounds display interesting one- or two-photon absorption properties. Their fluorescence emission wavelength and quantum yields (Phi) are dependent on the solvent polarity, with a red-shift in a more polar environment (lambda(max,em) = 460-467 nm, Phi > 0.53 in DMSO, and lambda(max,em) = 475-491 nm, Phi